Synthesis of Magnetic Microspheres with Immobilized Metal Ions for Enrichment and Direct Determination of Phosphopeptides by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry


  • The work was supported by grants from National Basic Research Priorities Programme (Project No. 2001CB510202), Shanghai Municipal Commission for Science and Technology (0452nm012), Shanghai Basic Research Priorities Programme (No. 05dz19741), and Natural Science Foundation of China (Project No. 39870451). Supporting Information is available online from Wiley InterScience or from the author.


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Core/shell structured magnetic silica microspheres with immobilized Fe3+ (see image) are prepared with a simple method. Using a magnetic field, the microspheres are quickly, efficiently, and specifically enriched. Phosphopeptide identification can then be achieved through mass spectroscopy. This is a new method for the enrichment of phosphopeptides and opens up the possibility of other new applications using the microspheres.