Guiding Cell Migration Using One-Way Micropattern Arrays


  • This work was supported by the National Institutes of Health (EB 003979).


original image

A single NIH 3T3 fibroblast on a teardrop island, surrounded by a cell-resistant background, extends lamellipodia from both sharp and blunt ends. However, the staggered arrangement of the islands and preferential extension of lamellipodia parallel to the cell body only permit attachment of lamellipodia extended from the blunt end, resulting in exclusive counterclockwise migration (the figure is ca. 200 μm wide).