Support for this work is provided by NIH (EB003447). We would like to thank Dr. Sing Yian Chew and I-Chien Liao for their assistance with the electrospinning protocol. Supporting Information is available online from Wiley InterScience or from the author.
Myogenic Induction of Aligned Mesenchymal Stem Cell Sheets by Culture on Thermally Responsive Electrospun Nanofibers†
Article first published online: 21 AUG 2007
Copyright © 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 19, Issue 19, pages 2775–2779, October, 2007
How to Cite
Dang, J. M. and Leong, K. W. (2007), Myogenic Induction of Aligned Mesenchymal Stem Cell Sheets by Culture on Thermally Responsive Electrospun Nanofibers. Adv. Mater., 19: 2775–2779. doi: 10.1002/adma.200602159
- Issue published online: 1 OCT 2007
- Article first published online: 21 AUG 2007
- Manuscript Revised: 23 JAN 2007
- Manuscript Received: 21 SEP 2006
- NIH. Grant Number: EB003447
- Composite materials;
- Stimuli-responsive materials
A thermally reversible culture substrate with a topographically active surface of aligned nanofibers is able to induce cytoskeletal alignment and nucleus elongation. These morphological changes induce myogenic differentiation in stem cells. The differentiated cells can be recovered in sheet form by thermally induced dissolution of the substrate. A surface able to provide topographical cues to create an aligned stem cell sheet, activated to differentiate to a specific lineage, can have a significant impact on engineering of tissue constructs for regenerative medicine applications.