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A Gold Nanoparticle-Based Aptamer Target Binding Readout for ATP Assay


  • J.W. and L.W. contributed equally to this work. This work was supported by National Natural Science Foundation (60537030 and 20725516), Shanghai Municipal Commission for Science and Technology (03DZ14025, 0652nm006 and 0652nm016), National Basic Research Program of China (2006CB933000 and 2007CB93600), Shanghai Rising-Star Program and Chinese Academy of Sciences. Supporting Information is available online from Wiley InterScience or from the authors.


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A gold nanoparticle-based detection strategy for ATP assays. In the absence of ATP, gold nanoparticles are not stabilized by the rigid duplex, thus they are readily aggregated by salt (solution displaying blue colors); in contrast ATP induces a duplex-to-aptamer structural switching, liberating a random coil-like ssDNA. Gold nanoparticles are stabilized by the liberated ssDNA, showing high resistance to salt-induced aggregation (solution staying in red).

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