Silk Fibroin Microfluidic Devices


  • The authors would like to acknowledge the following: The MEMS Technology Group at the Draper Laboratory for direct funding and use of facilities; James Hsiao and Asish Misra for contributions in microfabrication and cell culture; The Tissue Engineering Resource Center at Tufts University for providing cocoons; Hyeon Joo Kim for technical discussions and assistance in silk purification. C.J.B. was funded through a Draper Fellowship. Additional funding provided through DL-H-550154, and by NIH R01DE-013023-06. Support from the Center for Integration of Medicine and Innovative Technology, US Army DAMD17-02-2-0006, is also gratefully acknowledged. The content of this paper does not necessarily reflect the position or the policy of the government, and no official endorsement should be inferred. Supporting Information is available online from Wiley InterScience or from the authors.


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Microfluidic devices were fabricated from silk fibroin using an aqueous molding process adapted from soft lithography. The process results in mechanically robust, patent devices with macroscopic fluidic connections. Hepatocytes were seeded in the device and perfused for up to five days during which they exhibited liver-specific functions equivalent to that observed for standard tissue culture substrates.