Dynamic Materials Based on a Protein Conformational Change


  • This work was supported by the American Chemical Society Petroleum Research Fund (44235-G7). The authors thank Wei-Jen Tang for providing engineered CaM plasmids, and Milan Mrksich for helpful discussions. Supporting Information is available online from Wiley InterScience or from the authors.


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A protein-based dynamic hydrogel was generated via photo-crosslinking of an engineered protein-polymer conjugate. The protein calmodulin was used in this study due to its pronounced hinge motion in the presence of a variety of ligands. The resulting hydrogel is capable of undergoing substantial (>65%) and controllable volume transitions in the presence of calmodulin ligands, and the change can be attributed to a shift in protein conformation upon ligand binding.