Advanced Materials

Cationic Conjugated Polymer/DNA Complexes for Amplified Fluorescence Assays of Nucleases and Methyltransferases

Authors

  • F. Feng,

    1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080 (P.R. China)
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  • Y. Tang,

    1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080 (P.R. China)
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  • F. He,

    1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080 (P.R. China)
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  • M. Yu,

    1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080 (P.R. China)
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  • X. Duan,

    1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080 (P.R. China)
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  • S. Wang,

    1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080 (P.R. China)
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  • Y. Li,

    1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080 (P.R. China)
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  • D. Zhu

    1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080 (P.R. China)
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  • The authors are grateful for financial support from the “100 Talents” program of Chinese Academy of Sciences, the National Natural Science Foundation of China (20574073 and 20421101), the National Basic Research Program of China (No. 2006CB806200) and the National Major Research Plan of China (No. 2006CB932100). Supporting Information is available online from Wiley InterScience or from the authors.

Abstract

A new method is developed for sensitive, homogeneous, and convenient assays of nucleases and methyltransferases using complexes of cationic conjugated polymers with DNA containing a fluorescein tag at the 5′-terminus (DNA-Fl). The cleavage of DNA by nucleases can be monitored by fluorescence spectra by observing conjugated-polymer or fluorescein emission changes in aqueous solutions.

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