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Molded Nanowell Electrodes for Site-Selective Single Liposome Arrays

Authors

  • P. Kim,

    1. School of Mechanical and Aerospace Engineering and the Institute of Advanced Machinery and Design, Seoul National University, Seoul 151-742 (Korea)
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  • B. K. Lee,

    1. Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation, Honcho, Kawanguchi, 332-0012 Saitama (Japan)
    2. The Institute of Scientific and Industrial Research, Osaka University, Osaka 567-0047 (Japan)
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  • H. Y. Lee,

    1. Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation, Honcho, Kawanguchi, 332-0012 Saitama (Japan)
    2. The Institute of Scientific and Industrial Research, Osaka University, Osaka 567-0047 (Japan)
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  • T. Kawai,

    1. Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation, Honcho, Kawanguchi, 332-0012 Saitama (Japan)
    2. The Institute of Scientific and Industrial Research, Osaka University, Osaka 567-0047 (Japan)
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  • K. Y. Suh

    1. School of Mechanical and Aerospace Engineering and the Institute of Advanced Machinery and Design, Seoul National University, Seoul 151-742 (Korea)
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  • This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund) (KRF-2006-003-D00040) and the Micro Thermal System Research Center of Seoul National University. This work was also supported by Core Research for Evolutional Science and Technology (CREST) of Japan Science and Technology Corporation. The authors are grateful to the Minuta Tech. for providing polyurethane acrylate materials.

Abstract

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We present a nanowell (NW)-based biosensing platform for electrochemically detecting biological reactions on the level of single lipid vesicle. The NW geometry of a gently-sloped vertical wall is found to be optimal for selective deposition of single liposomes without capillary resistances. Also, the use of a hydrophilic, non-biofouling PEG copolymer is essential to provide distinctively separated FLV arrays without nonspecific adsorption.

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