Long-Lived Planar Lipid Bilayer Membranes Anchored to an In Situ Polymerized Hydrogel

Authors

  • N. Malmstadt,

    1. Department of Bioengineering and California Nanosystems Institute, University of California, Los Angeles, 7523 Boelter Hall, Los Angeles, CA, 90095-1600 (USA)
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  • T.-J. Jeon,

    1. Department of Bioengineering and California Nanosystems Institute, University of California, Los Angeles, 7523 Boelter Hall, Los Angeles, CA, 90095-1600 (USA)
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  • J. J. Schmidt

    1. Department of Bioengineering and California Nanosystems Institute, University of California, Los Angeles, 7523 Boelter Hall, Los Angeles, CA, 90095-1600 (USA)
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  • N.M. and T-J.J. contributed equally to this work. Confocal FRAP was performed at the Advanced Light Microscopy/Spectroscopy Shared Facility in the California NanoSystems Institute at UCLA. We thank Stephen Cheley for the kind donation of αHL. Funding was provided by UCLA FRG, ACS PRF (PRF#42947-G5), and NSF CAREER (0644442). Supporting Information is available online from Wiley InterScience or from the authors.

Abstract

original image

Bilayer membranes that are covalently conjugated in situ to an encapsulating hydrogel have been formed. These membranes are extremely long-lived, lasting over ten days in standard laboratory conditions, and are sufficiently fluid to support protein incorporation.

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