Thermoresponsive Microtextured Culture Surfaces Facilitate Fabrication of Capillary Networks

Authors

  • Y. Tsuda,

    1. Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawadacho, Shinjuku, Tokyo 162-8666 (Japan)
    2. Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8571 (Japan)
    3. Biomaterials Center, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044 (Japan)
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  • M. Yamato,

    1. Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawadacho, Shinjuku, Tokyo 162-8666 (Japan)
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  • A. Kikuchi,

    1. Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawadacho, Shinjuku, Tokyo 162-8666 (Japan)
    2. Present address: Department of Materials Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda-shi, Chiba 278-8510, Japan
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  • M. Watanabe,

    1. Research & Development Center, Dai Nippon Printing Co., Ltd., 250-1 Wakashiba, Kashiwa, Chiba 277-0871 (Japan)
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  • G. Chen,

    1. Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8571 (Japan)
    2. Biomaterials Center, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044 (Japan)
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  • Y. Takahashi,

    1. Research & Development Center, Dai Nippon Printing Co., Ltd., 250-1 Wakashiba, Kashiwa, Chiba 277-0871 (Japan)
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  • T. Okano

    1. Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawadacho, Shinjuku, Tokyo 162-8666 (Japan)
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  • The authors appreciate the useful comments and technical criticism from J. Yang (Tokyo Women's Medical University) and Dr. B. C. Isenberg (Boston University). The authors thank Y. Shigematsu (Tokyo Women's Medical University) and Prof. M. Wada (Nara Women's University) for their technical assistance with confocal laser scanning microscopy. The authors also acknowledge T. Tonegawa (National Institute for Materials Science, Japan) for his technical assistance with the SEM observations. The present research was supported by Formation of Innovation Center for Fusion of Advanced Technologies in the Special Coordination Funds for Promoting Science and Technology from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. Y.T. acknowledges support from a Japan Society for Promotion of Science Research Fellowship (Project No. 17-7231).

Abstract

original image

A novel cell culture surface is developed that induces the tubular formation of endothelial cells and facilitates the intact harvesting of fabricated capillary-like structures by utilizing a thermoresponsive polymer, poly(N-isopropylacrylamide). The tubular cell networks, such as the one shown in the figure, may be applicable for the fabrication of 3D vascularized tissue grafts.

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