Ultrathin Multilayered Films Assembled from “Charge-Shifting” Cationic Polymers: Extended, Long-Term Release of Plasmid DNA from Surfaces


  • Financial support was provided by the 3M Corporation and the Arnold and Mabel Beckman Foundation. We thank Dr. Steven M. Heilmann (3M) for providing samples of poly(2-vinyl-4,4-dimethylazlactone) and Maren Buck for assistance with polymer synthesis and many helpful discussions, and we are grateful to the NSF (CHE-9208463) and the NIH (NIH 1 S10 RR0 8389-01) for support of the UW NMR spectroscopy facilities. D.M.L. is an Alfred P. Sloan Research Fellow. Supporting Information is available online from Wiley InterScience or from the authors.


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Ultrathin multilayered films can be designed to release plasmid DNA for three months by using side-chain functionalized ‘charge-shifting' cationic polymers. Slow hydrolysis of the side chains of these polymers facilitates film disruption and release of DNA over periods longer than films fabricated using degradable cationic polymers, and suggests approaches to the fabrication of thin films for the localized and long-term release of DNA, proteins, or other agents from surfaces.