Direct Delivery and Submicrometer Patterning of DNA by a Nanofountain Probe


  • This work was supported primarily by the Nanoscale Science and Engineering Initiative of the National Science Foundation under NSF Award Number EEC–0118025. HDE acknowledges the support provided by the National Science Foundation through NIRT project No. CMS00304472. CAM acknowledges the AFOSR, ARO, and DARPA for generous support. CAM is also grateful for a NIH Director's Pioneer Award. The authors acknowledge the Microfabrication Applications Laboratory (MAL) of the University of Illinois at Chicago, the Cornell NanoScale Science & Technology Facility (CNF) of Cornell University, the Materials Processing and Crystal Growth Facility (MPCGF) of Northwestern University, Northwestern University Atomic- and Nanoscale Characterization Experimental Center (NUANCE), and the Electron Microscopy Center (EMC) of Argonne National Laboratory for the fabrication and characterization facilities.


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Biomolecular patterning is achieved using a nanofountain probe (NFP) that delivers a liquid solution of DNA through a volcano-like aperture to a substrate (see figure). Spot features as small as 200 nm in diameter can be routinely made at room temperature over a wide humidity range. Gold nanoparticles functionalized with sequences complementary to the patterned DNA demonstrate that biological activity is retained by the deposited features.