Synthesis of Functionalized Au Nanoparticles for Protein Detection


  • The authors would like to thank Dr. S. T. Selvan for helpful discussion and Dr. Lan Zhao for TEM characterization. This work was supported by the Institute of Bioengineering and Nanotechnology (Biomedical Research Council, Agency for Science, Technology and Research, Singapore). Supporting information is available online from Wiley InterScience or from the author.


original image

Aptamer and antibody functionalized Au nanoparticles are synthesized and used for protein detection (see figure). These particles are highly water soluble and as small as 10 nm. However, they are enlarged after a protein binding event to enhance signal sensitivity. A conventional western blot protocol is used to enable detection of the proteins with nanomolar sensitivity, with the naked eye.