Highly Emissive Self-assembled Organic Nanoparticles having Dual Color Capacity for Targeted Immunofluorescence Labeling

Authors

  • Hyong-Jun Kim,

    1. Department of Materials Science and Engineering, University of Michigan, Ann Arbor, MI 48109 (USA)
    2. Current address: OLED center, School of Materials Science and Engineering, Seoul National University, Seoul, 151-744, Korea.
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  • Jiseok Lee,

    1. Macromolecular Science and Engineering, University of Michigan, Ann Arbor, MI 48109 (USA)
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  • Tae-Hoon Kim,

    1. Department of Materials Science and Engineering, University of Michigan, Ann Arbor, MI 48109 (USA)
    2. Department of Advanced Organic Materials and Textile System, Engineering Chungnam National University, Daejon, 305-764 (Korea)
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  • Taek Seung Lee,

    1. Department of Advanced Organic Materials and Textile System, Engineering Chungnam National University, Daejon, 305-764 (Korea)
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  • Jinsang Kim

    Corresponding author
    1. Department of Materials Science and Engineering, University of Michigan, Ann Arbor, MI 48109 (USA)
    2. Chemical Engineering and Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109 (USA)
    3. Macromolecular Science and Engineering, University of Michigan, Ann Arbor, MI 48109 (USA)
    • Department of Materials Science and Engineering, University of Michigan, Ann Arbor, MI 48109 (USA).
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  • H.-J.K. and J.L. contributed equally to this work. This research was supported by the National Science Foundation (BES 0428010), NSF CAREER Award (DMR 0644864), and the Center for Chemical Genomics-Thermo Fisher Grant. T.-H.K. acknowledges Korea Science and Engineering Foundation (KOSEF) for the exchange student support. Supporting Information is available online from Wiley InterScience or from the authors.

Abstract

original image

A new benzoxazole molecule has been synthesized and self-assembled with functionalized diacetylene molecules to obtain well-defined nanoparticles of 80 nm diameter. The nanoparticles show enhanced fluorescence emission of 38% quantum yield compared to 3% in THF solution. Selective targeting and dual color visualization of patterned avidin arrays were achieved by using these nanoparticles to demonstrate their promising application for immunofluorescence labeling.

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