Direct Visualization of Glucose Phosphorylation with a Cationic Polythiophene

Authors

  • Y. Tang,

    1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080 (P.R. China)
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  • F. Feng,

    1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080 (P.R. China)
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  • M. Yu,

    1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080 (P.R. China)
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  • L. An,

    1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080 (P.R. China)
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  • F. He,

    1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080 (P.R. China)
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  • S. Wang,

    1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080 (P.R. China)
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  • Y. Li,

    1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080 (P.R. China)
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  • D. Zhu,

    1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080 (P.R. China)
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  • G. C. Bazan

    1. Departments of Chemistry and Materials, Institute of Polymers and Organic Solids, University of California, Santa Barbara, CA 93106-9510 (USA)
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  • The authors are grateful for the financial support from the “100 Talents” program of the Chinese Academy of Sciences, the National Natural Science Foundation of China (20574073 and 20421101), the National Basic Research Program of China (2006CB806200), the National Major Research Plan of China (2006CB932100), and the U.S. National Science Foundation (DMR 0606414).

Abstract

original image

ATP-dependent phosphorylation of glucose catalyzed by hexokinase can be detected in real time by a homogeneous visual inspection based on a water-soluble cationic polythiophene derivative (CPT) that responds to the presence of ATP. The color of CPT solutions changes from pink-red in the presence of ATP to yellow when ATP is converted to ADP following the glucose phosphorylation by hexokinase.

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