Non-Lithographic Wrinkle Nanochannels for Protein Preconcentration

Authors

  • Seok Chung,

    1. Germeshausen Professor of Mechanical and Biological Engineering Department of Mechanical Engineering and Biological Engineering Massachusetts Institute of Technology Cambridge, MA 02139 (USA)
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  • Jeong Hoon Lee,

    1. Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology Cambridge, MA 02139 (USA)
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  • Myoung-Woon Moon,

    1. Division of Engineering and Applied Sciences Harvard University Cambridge, MA 02138 (USA)
    2. Future Fusion Technology Laboratory Korea Institute of Science and Technology 136-791 (Korea)
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  • Jongyoon Han,

    1. Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology Cambridge, MA 02139 (USA)
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  • Roger D. Kamm

    Corresponding author
    1. Germeshausen Professor of Mechanical and Biological Engineering Department of Mechanical Engineering and Biological Engineering Massachusetts Institute of Technology Cambridge, MA 02139 (USA)
    • Germeshausen Professor of Mechanical and Biological Engineering Department of Mechanical Engineering and Biological Engineering Massachusetts Institute of Technology Cambridge, MA 02139 (USA).
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  • S.C. was supported by an IT Scholarship Program supervised by the IITA (Institute for Information Technology Advancement) & MIC (Ministry of Information and Communication), Korea. J.H.L. received support by NIH grants R01-EB005743, P30-ES002109-28 (MIT-CEHS), and a Korea Research Foundation grant (KRF-2005-000-10051-0).

Abstract

original image

A simple, non-lithographic method to create a nanofluidic channel array that exploits wrinkles is demonstrated. The dimension and position of the wrinkles can be precisely controlled, producing wrinkle nanochannels (WNCs) that range in size from several tens of nanometers to micrometers. The usefulness of the WNCs is demonstrated by preconcentration of a protein, which is shown to vary linearly. Preconcentration levels of more than 100 can be achieved within 10 minutes.

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