Toward the Accurate Read-out of Quantum Dot Barcodes: Design of Deconvolution Algorithms and Assessment of Fluorescence Signals in Buffer

Authors

  • J. A. Lee,

    1. Institute of Biomaterials and Biomedical Engineering, Department of Materials Science and Engineering, The Centre for Cellular and Biomolecular Research, University of Toronto, 160 College St., Toronto, ON M5S 3E1 (Canada)
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  • A. Hung,

    1. Institute of Biomaterials and Biomedical Engineering, Department of Materials Science and Engineering, The Centre for Cellular and Biomolecular Research, University of Toronto, 160 College St., Toronto, ON M5S 3E1 (Canada)
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  • S. Mardyani,

    1. Institute of Biomaterials and Biomedical Engineering, Department of Materials Science and Engineering, The Centre for Cellular and Biomolecular Research, University of Toronto, 160 College St., Toronto, ON M5S 3E1 (Canada)
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  • A. Rhee,

    1. Institute of Biomaterials and Biomedical Engineering, Department of Materials Science and Engineering, The Centre for Cellular and Biomolecular Research, University of Toronto, 160 College St., Toronto, ON M5S 3E1 (Canada)
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  • J. Klostranec,

    1. Institute of Biomaterials and Biomedical Engineering, Department of Materials Science and Engineering, The Centre for Cellular and Biomolecular Research, University of Toronto, 160 College St., Toronto, ON M5S 3E1 (Canada)
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  • Y. Mu,

    1. National Laboratory of Industry Control Techniques, Zhejiang University, Hangzhou, Zhejiang 310027 (P.R. China)
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  • D. Li,

    1. Institute of Biomaterials and Biomedical Engineering, Department of Materials Science and Engineering, The Centre for Cellular and Biomolecular Research, University of Toronto, 160 College St., Toronto, ON M5S 3E1 (Canada)
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  • W. C. W. Chan

    1. Institute of Biomaterials and Biomedical Engineering, Department of Materials Science and Engineering, The Centre for Cellular and Biomolecular Research, University of Toronto, 160 College St., Toronto, ON M5S 3E1 (Canada)
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  • This project was funded by Genome Canada through the Ontario Genome Institute. Y.M. acknowledges CSC (22822101) and NSFC (30200050) for visiting fellow support. J.K. and S.M. acknowledge NSERC for student fellowships.

Abstract

original image

Signal processing methods and constraints for discerning the fluorescence signals of the QD-barcodes are explored. QD-barcodes and their corresponding fluorescence spectra (see figure) require signal processing algorithms in order to be uniquely identified. Using these algorithms, we determined the number of available barcodes for use in biological detection. We also studied the impact of chemical constraints such as buffer and pH level on the barcode and read-out design.

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