SEARCH

SEARCH BY CITATION

Keywords:

  • Arrays;
  • Biocompatible materials;
  • Lithography;
  • Proteins;
  • Self-assembly
Thumbnail image of graphical abstract

Electron-induced chemical lithography combined with self-assembled monolayers and multivalent chelators for high-affinity capturing of His-tagged proteins are used to obtain specific, stable, highly parallel, and functional protein micro- and nanoarrays on solid substrates. The functionality of the generated large-area protein arrays is shown in situ via specific, homogeneous, oriented and reversible immobilization of His6-tagged 20S proteasome and fluorescence labelled His10-tagged maltose binding proteins.