The grant support of BMBF and DFG is acknowledged. Part of this work was performed at the Swiss Light Source, Paul Scherrer Institut, Villigen, Switzerland. Supporting Information is available online from Wiley InterScience or from the authors.
Molecular Self-Assembly, Chemical Lithography, and Biochemical Tweezers: A Path for the Fabrication of Functional Nanometer-Scale Protein Arrays†
Article first published online: 17 JAN 2008
Copyright © 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 20, Issue 3, pages 471–477, February, 2008
How to Cite
Turchanin, A., Tinazli, A., El-Desawy, M., Großmann, H., Schnietz, M., Solak, H. H., Tampé, R. and Gölzhäuser, A. (2008), Molecular Self-Assembly, Chemical Lithography, and Biochemical Tweezers: A Path for the Fabrication of Functional Nanometer-Scale Protein Arrays. Adv. Mater., 20: 471–477. doi: 10.1002/adma.200702189
- Issue published online: 5 FEB 2008
- Article first published online: 17 JAN 2008
- Manuscript Revised: 26 OCT 2007
- Manuscript Received: 29 AUG 2007
- Biocompatible materials;
Electron-induced chemical lithography combined with self-assembled monolayers and multivalent chelators for high-affinity capturing of His-tagged proteins are used to obtain specific, stable, highly parallel, and functional protein micro- and nanoarrays on solid substrates. The functionality of the generated large-area protein arrays is shown in situ via specific, homogeneous, oriented and reversible immobilization of His6-tagged 20S proteasome and fluorescence labelled His10-tagged maltose binding proteins.