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Molecular Self-Assembly, Chemical Lithography, and Biochemical Tweezers: A Path for the Fabrication of Functional Nanometer-Scale Protein Arrays


  • The grant support of BMBF and DFG is acknowledged. Part of this work was performed at the Swiss Light Source, Paul Scherrer Institut, Villigen, Switzerland. Supporting Information is available online from Wiley InterScience or from the authors.


Electron-induced chemical lithography combined with self-assembled monolayers and multivalent chelators for high-affinity capturing of His-tagged proteins are used to obtain specific, stable, highly parallel, and functional protein micro- and nanoarrays on solid substrates. The functionality of the generated large-area protein arrays is shown in situ via specific, homogeneous, oriented and reversible immobilization of His6-tagged 20S proteasome and fluorescence labelled His10-tagged maltose binding proteins.

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