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Small-Molecule-Directed Assembly: A Gold Nanoparticle-Based Strategy for Screening of Homo-Adenine DNA Duplex Binders

Authors

  • G. Song,

    1. Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 (P.R. China)
    2. , Graduate School of the Chinese Academy of Sciences, Beijing 100039 (P.R. China)
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  • C. Chen,

    1. Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 (P.R. China)
    2. , Graduate School of the Chinese Academy of Sciences, Beijing 100039 (P.R. China)
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  • X. Qu,

    1. Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 (P.R. China)
    2. , Graduate School of the Chinese Academy of Sciences, Beijing 100039 (P.R. China)
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  • D. Miyoshi,

    1. Frontier Institute for Biomolecular Engineering Research (FIBER) Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501 (Japan)
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  • J. Ren,

    1. Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 (P.R. China)
    2. , Graduate School of the Chinese Academy of Sciences, Beijing 100039 (P.R. China)
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  • N. Sugimoto

    1. Frontier Institute for Biomolecular Engineering Research (FIBER) Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501 (Japan)
    2. Department of Chemistry, Faculty of Science and Engineering, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501 (Japan)
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  • Financial support was provided by the National Natural Science Foundation of China (Grants 20331020, 20325101, and 20473084), the Fund from CAS, and Grants-in-Aid and the “Academic Frontier” Project (2004-2009) from MEXT, Japan. Supporting Information is available online from Wiley InterScience or from the authors.

Abstract

By using AuNP-modified homo-adenine DNA conjugate as a model system, simple colorimetric and resonance Rayleigh scattering assays have been developed for screening small molecules that trigger the formation of the non-Watson–Crick homo-adenine duplexes. The assay presented here is more simplified in format as it involves only one type of ssDNA modified Au-NP, and can be easily adapted to high-throughput screening.

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