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Identification of a Highly Specific Hydroxyapatite-binding Peptide using Phage Display

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  • The authors gratefully acknowledge the support of the National Institute of Standards and Technology, in funding this work through Innovation in Measurement Science awards in Carbon Nanotubes Metrology and Cellular Biometrology. We also acknowledge the support of the National Research Council – National Institute of Standards and Technology postdoctoral fellowships (M.D.R and S.K.S.). The authors would also like to thank Gary E. Schumacher, D.D.S, M.S. of the American Dental Association for providing tooth samples. This article, a contribution of the National Institute of Standards and Technology, is not subject to US copyright. Certain equipment and instruments or materials are identified in the paper to adequately specify the experimental details. Such identification does not imply recommendation by the National Institute of Standards and Technology, nor does it imply the materials are necessarily the best available for the purpose.

Abstract

A peptide sequence SVSVGMKPSPRP that selectively recognizes hydroxyapatite (HA) is identified by using a phage display approach. The engineered sequence exhibits chemical and structural specificity for HA over calcium carbonate and HA's amorphous calcium phosphate precursor. In situ binding to HA in a tooth cross section further demonstrates the sequence specificity and utility in nondestructive imaging applications.

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