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Human Embryoid Bodies Containing Nano- and Microparticulate Delivery Vehicles

Authors

  • Lino Ferreira,

    1. Department of Chemical Engineering, Massachusetts Institute of Technology, E25-342 MIT, 77 Massachusetts Avenue, Cambridge, MA 02139 (USA)
    2. Center of Neurosciences and Cell Biology, University of Coimbra, 3004-517 Coimbra (Portugal)
    3. Biocant – Biotechnology Innovation Center, 3060-197 Cantanhede (Portugal)
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  • Trevor Squier,

    1. Department of Chemical Engineering, Massachusetts Institute of Technology, E25-342 MIT, 77 Massachusetts Avenue, Cambridge, MA 02139 (USA)
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  • Hyoungshin Park,

    1. Department of Chemical Engineering, Massachusetts Institute of Technology, E25-342 MIT, 77 Massachusetts Avenue, Cambridge, MA 02139 (USA)
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  • Hannah Choe,

    1. Department of Chemical Engineering, Massachusetts Institute of Technology, E25-342 MIT, 77 Massachusetts Avenue, Cambridge, MA 02139 (USA)
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  • Daniel S. Kohane,

    1. Department of Chemical Engineering, Massachusetts Institute of Technology, E25-342 MIT, 77 Massachusetts Avenue, Cambridge, MA 02139 (USA)
    2. Laboratory for Biomaterials and Drug Delivery, Department of Anesthesia, Division of Critical Care Medicine, Children's Hospital Boston, 300 Longwood Ave., Boston, MA 02115 (USA)
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  • Robert Langer

    Corresponding author
    1. Department of Chemical Engineering, Massachusetts Institute of Technology, E25-342 MIT, 77 Massachusetts Avenue, Cambridge, MA 02139 (USA)
    • Department of Chemical Engineering, Massachusetts Institute of Technology, E25-342 MIT, 77 Massachusetts Avenue, Cambridge, MA 02139 (USA).
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  • The authors would like to thank Jason Fuller and Jon Aanestad for valuable suggestions and the financial support of Fundação para a Ciência e a Tecnologia (SFRH/BPD/14502/2003; fellowship to L.F), NIH (grants HL060435, DE13023 and HL076485) and the MIT–Portugal Program (focus in Bioengineering). Supporting Information is available online from Wiley InterScience or from the authors.

Abstract

Differentiation of human embryonic stem cells is affected by the incorporation of growth-factor-releasing particles in embryoid bodies. Incorporation of these particles has a minimal effect on cell viability and proliferation but a large impact on differentiation. The figure shows the distribution of TRITC-labeled microparticles in embryoid bodies, as evaluated by confocal microscopy.

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