Polyelectrolyte Films Boost Progenitor Cell Differentiation into Endothelium-like Monolayers

Authors

  • Nicolas Berthelemy,

    1. Group of Bioengineering (LEMTA-UMR CNRS 7563) UHP-Nancy 1, Faculté de Médecine 9, avenue de la forêt de Haye 54505 Vandoeuvre-lès-Nancy (France)
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  • Halima Kerdjoudj,

    1. Group of Bioengineering (LEMTA-UMR CNRS 7563) UHP-Nancy 1, Faculté de Médecine 9, avenue de la forêt de Haye 54505 Vandoeuvre-lès-Nancy (France)
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  • Caroline Gaucher,

    1. Group of Bioengineering (LEMTA-UMR CNRS 7563) UHP-Nancy 1, Faculté de Médecine 9, avenue de la forêt de Haye 54505 Vandoeuvre-lès-Nancy (France)
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  • Pierre Schaaf,

    1. Institut Charles Sadron (UPR 22, CNRS) 6, rue Boussingault 67083 Strasbourg Cedex (France)
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  • Jean-François Stoltz,

    1. Group of Bioengineering (LEMTA-UMR CNRS 7563) UHP-Nancy 1, Faculté de Médecine 9, avenue de la forêt de Haye 54505 Vandoeuvre-lès-Nancy (France)
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  • Patrick Lacolley,

    1. Institut National de la Santé et de la Recherche Médicale, INSERM Unité 684 UHP-Nancy 1, Faculté de Médecine 9, avenue de la forêt de Haye 54505 Vandoeuvre-lès-Nancy (France)
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  • Jean-Claude Voegel,

    1. Institut National de la Santé et de la Recherche Médicale, INSERM Unité 595 ULP-Strasbourg, Faculté de Chirurgie Dentaire 11, rue Humann 67085 Strasbourg Cedex (France)
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  • Patrick Menu

    Corresponding author
    1. Group of Bioengineering (LEMTA-UMR CNRS 7563) UHP-Nancy 1, Faculté de Médecine 9, avenue de la forêt de Haye 54505 Vandoeuvre-lès-Nancy (France)
    • Group of Bioengineering (LEMTA-UMR CNRS 7563) UHP-Nancy 1, Faculté de Médecine 9, avenue de la forêt de Haye 54505 Vandoeuvre-lès-Nancy (France).===

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  • N. Berthelemy and H. Kerdjoudj contributed equally to this work. This work was partly supported by Région de Lorraine. Supporting Information is available online from Wiley InterScience or from the authors.

Abstract

original image

Rapid differentiation of endothelial progenitor cells (EPCs) into confluent mature endothelial cells (see figure) is important in tissue engineering for the design of autologous, nonthrombotic, vascular grafts. A new method based on EPC culture on poly(sodium-4- styrene-sulfonate)/poly(allylamine hydrochloride), that is, polyelectrolyte-multilayer-coated substrates, reduces the time from two months to two weeks.

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