Communication

Uptake of Colloidal Polyelectrolyte-Coated Particles and Polyelectrolyte Multilayer Capsules by Living Cells†

  1. Alumdena Muñoz Javier1,2,
  2. Oliver Kreft3,
  3. Maximilian Semmling2,4,
  4. Susanne Kempter1,
  5. Andre G. Skirtach3,
  6. Oliver T. Bruns5,
  7. Pablo del Pino2,
  8. Mathieu F. Bedard3,
  9. Joachim Rädler1,
  10. Josef Käs4,
  11. Christian Plank6,
  12. Gleb B. Sukhorukov3,7,
  13. Wolfgang J. Parak1,2,*

Article first published online: 21 AUG 2008

DOI: 10.1002/adma.200703190

Issue

Advanced Materials

Advanced Materials

Volume 20, Issue 22, pages 4281–4287, November 18, 2008

Additional Information

How to Cite

Muñoz Javier, A., Kreft, O., Semmling, M., Kempter, S., Skirtach, A. G., Bruns, O. T., del Pino, P., Bedard, M. F., Rädler, J., Käs, J., Plank, C., Sukhorukov, G. B. and Parak, W. J. (2008), Uptake of Colloidal Polyelectrolyte-Coated Particles and Polyelectrolyte Multilayer Capsules by Living Cells. Adv. Mater., 20: 4281–4287. doi: 10.1002/adma.200703190

Author Information

  1. 1

    Center for Nanoscience, Ludwig Maximilians Universität München München (Germany)

  2. 2

    Fachbereich Physik, Philipps Universität Marburg, Marburg (Germany)

  3. 3

    Max-Planck-Institut für Kolloid- und Grenzflächenforschung, Golm (Germany)

  4. 4

    Universität Leipzig, Leipzig (Germany)

  5. 5

    Department of Biochemistry and Molecular Biology II: Molecular Cell Biology University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg (Germany)

  6. 6

    Technische Universität München, München (Germany)

  7. 7

    School of Engineering and Materials Sciences, Queen Mary University of London, London (UK)

*Center for Nanoscience, Ludwig Maximilians Universität München München (Germany).

  1. †

    The authors are grateful to Dr. Pilar Rivera Gil, Prof. Sebastian Springer, and to Prof. Mathias Winterhalter for helpful discussions and to Annegret Praast for microcapsule preparation. The work was supported by the European Union (Nanointeract project) and the Deutsche Forschungsgesellschaft (PA 794/4-1). Oliver Bruns is supported by a fellowship from the Studienstiftung des Deutschen Volkes. Supporting Information is available online from Wiley InterScience or from the author.

Publication History

  1. Issue published online: 13 NOV 2008
  2. Article first published online: 21 AUG 2008
  3. Manuscript Revised: 28 FEB 2008
  4. Manuscript Received: 24 DEC 2007

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Keywords:

  • confocal microscopy;
  • fluorescence microscopy;
  • metal nanoparticles;
  • polymeric materials;
  • transmission electron microscopy
Thumbnail image of graphical abstract

Rigid particles as well as soft capsules can be ingested by cells and stored in acidic compartments around the nucleus. TEM and fluorescence images show that the rigid particles retain their original spherical shape whereas the hollow and thus more flexible capsules are deformed and squeezed upon the incorporation process. Though soft capsules are deformed upon uptake, the cargo loaded into the capsules is not released into the cytosol.

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