Inside Front Cover: Combinatorial Modification of Degradable Polymers Enables Transfection of Human Cells Comparable to Adenovirus (Adv. Mater. 19/2007)

Authors

  • J. J. Green,

    1. Division of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 (USA)
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  • G. T. Zugates,

    1. Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 (USA)
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  • N. C. Tedford,

    1. Division of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 (USA)
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  • Y.-H. Huang,

    1. Lankenau Institute for Medical Research, 100 East Lancaster Avenue, Wynnewood PA 19096 (USA)
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  • L. G. Griffith,

    1. Division of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 (USA)
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  • D. A. Lauffenburger,

    1. Center for Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 (USA)
    2. Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 (USA)
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  • J. A. Sawicki,

    1. Lankenau Institute for Medical Research, 100 East Lancaster Avenue, Wynnewood PA 19096 (USA)
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  • R. Langer,

    1. Center for Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 (USA)
    2. Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 (USA)
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  • D. G. Anderson

    1. Center for Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 (USA)
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Abstract

On p. 2836, Daniel G. Anderson and co-workers report the development of end-modified poly(β-amino ester)s that are able to deliver DNA to primary human umbilical vein endothelial cells at levels comparable to adenovirus at a Multiplicity of Infection between 100 and 500, and two orders of magnitude better than the commonly used non-viral polymeric vector, poly(ethylene imine). Small structural changes were found to have dramatic effects on multiple steps of gene delivery including the DNA binding affinity, nanoparticle size, intracellular DNA uptake, and final protein expression. In vivo, these polymer modifications enhance DNA delivery to ovarian tumors.

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