Advanced Materials

Synthesis of Complexable Fluorescent Superparamagnetic Iron Oxide Nanoparticles (FL SPIONs) and Cell Labeling for Clinical Application

Authors

  • Jae-Ho Lee,

    Corresponding author
    1. Experimental Neuroimaging Section Laboratory of Diagnostic Radiology Research National Institutes of Health Bethesda, MD 20892-1074 (USA)
    • Experimental Neuroimaging Section Laboratory of Diagnostic Radiology Research National Institutes of Health Bethesda, MD 20892-1074 (USA).
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  • Benjamin Schneider,

    1. Department of Chemical Engineering, University of Maryland College Park, MD 20742-2111 (USA)
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  • Elaine K. Jordan,

    1. Experimental Neuroimaging Section Laboratory of Diagnostic Radiology Research National Institutes of Health Bethesda, MD 20892-1074 (USA)
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  • Wei Liu,

    1. Experimental Neuroimaging Section Laboratory of Diagnostic Radiology Research National Institutes of Health Bethesda, MD 20892-1074 (USA)
    2. Philips Research North America Briarcliff Manor, NY 10510 (USA)
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  • Joseph A. Frank

    1. Experimental Neuroimaging Section Laboratory of Diagnostic Radiology Research National Institutes of Health Bethesda, MD 20892-1074 (USA)
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  • This research was supported by the Intramural Research Program of the Clinical Center at the National Institutes of Health. The authors would like to acknowledge the help of Dr. Leapman for TEM analysis. Supporting Information is available online from Wiley InterScience or from the authors.

Abstract

We describe a new synthesis route for fluorescent superparamagnetic iron oxide nanoparticles (FL SPIONs) and the self-assembled complex formed using FL SPION and protamine sulfate for cell labeling. This synthesis route involves the conjugation of the FDA-approved dextran-coated ferumoxides with fluorescent dextran. The novel nanostructure of FL SPION is stable in vitro and it complexes with a cationic peptide.

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