Ultrathin Coatings with Change in Reactivity over Time Enable Functional In Vitro Networks Of Insect Neurons

Authors


  • A.R. and P.G. contributed equally. The authors want to thank Dr. Krystyna Albrecht for SFM measurements and Dr. Kristin E. Michael for evaluation of the manuscript. The German Science Foundation (DFG, graduate school 1035 “Biointerface”, Project B1 in the TR-SFB 37 and SPP 1259 “Intelligent Hydrogels”) and the EU (project “CILIA”, contract 016039, www.cilia-bioincs.org) are acknowledged for funding. SusTech GmbH & Co KG is acknowledged for support with the coating technology. Supporting Information is available online from Wiley InterScience or from the authors.

Abstract

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It's just not cricket! A novel coating system that enables covalent attachment of biomolecules in a nonfouling environment without use of additional chemical crosslinkers is presented. Concanavalin A is patterned on the coatings to direct cell adhesion and growth of neurons from the cricket Gryllus bimaculatus and generate functional, patterned in vitro insect neuronal networks for the first time.

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