Communication

Combinatorial Screening of Biomimetic Protein Affinity Materials†

  1. Trent R. Northen2,
  2. Matthew P. Greving1,
  3. Neal W. Woodbury1,*

Article first published online: 15 OCT 2008

DOI: 10.1002/adma.200800567

Issue

Advanced Materials

Advanced Materials

Volume 20, Issue 24, pages 4691–4697, December 17, 2008

Additional Information

How to Cite

Northen, T. R., Greving, M. P. and Woodbury, N. W. (2008), Combinatorial Screening of Biomimetic Protein Affinity Materials. Adv. Mater., 20: 4691–4697. doi: 10.1002/adma.200800567

Author Information

  1. 1

    Department of Chemistry and Biochemistry Biodesign Institute, Arizona State University Tempe, AZ 852 87 (USA)

  2. 2

    Life Sciences Division Lawrence Berkeley National Laboratory 1 Cyclotron Road, Berkeley, CA 94720 (USA)

*Department of Chemistry and Biochemistry Biodesign Institute, Arizona State University Tempe, AZ 85287 (USA).

  1. †

    T.R.N. and M.P.G. contributed equally to this work, and were both supported by NSF IGERT Fellowships. This work was funded by The Biodesign Institute at Arizona State University, Department of Energy (grant DE-FC36-05GO15016 and DE-AC02-05CH11231), NSF (grant DGE-0114434), and NSF grant no. CHE-0131222 (mass spectrometer). We would also like to thank Paul Belcher for the SPR measurements, Rashaad Sidique, and the Center for Solid State Science at Arizona State University for SEM images. Supporting Information is available online from Wiley InterScience or from the authors.

Publication History

  1. Issue published online: 16 DEC 2008
  2. Article first published online: 15 OCT 2008
  3. Manuscript Revised: 14 MAY 2008
  4. Manuscript Received: 27 FEB 2008

Funded by

  • Biodesign Institute at Arizona State University
  • Department of Energy. Grant Numbers: DE-FC36-05GO15016, DE-AC02-05CH11231
  • NSF. Grant Number: DGE-0114434
  • NSF. Grant Number: CHE-0131222

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Keywords:

  • arrays;
  • biomimetics;
  • gels;
  • polymers
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A patterned combinatorial library of peptide-grafted polymer affinity materials is screened for target protein binding. In situ light-directed synthesis is used to produce high-density libraries that allow for the screening in parallel within an area less than 1 cm2. From these libraries, specific peptide/polymer combinations are identified with very high target protein affinity, enabling facile detection of the target at concentrations in the low picomolar range in the presence of excess competitor.

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