Get access

3D Cell-Migration Studies using Two-Photon Engineered Polymer Scaffolds


  • Several people contributed to the work described in this paper. P.T. conceived of the basic idea for this work, designed and carried out the experiments, and analyzed the results. T.B. provided expertise on two-photon polymerization and polymer chemistry. C.M. assisted with the experiments. D.M. and E.M. supervised the research, analysis of the data, and the development of the manuscript. P.T. wrote the first draft of the manuscript; all authors subsequently took part in the revision process and approved the final copy of the manuscript. M. Winkler, J. Dowd and C. Evans provided feedback on the manuscript throughout its development. The research described in this paper was supported by the NSF-sponsored Materials Research Science and Engineering Center under contract DMR-0213805 and NIH under contract R37 DE013033. Supporting Information is available online from Wiley InterScience or from the author.


We use two-photon polymerization to fabricate 3D scaffolds with precise control over pore size and shape for studying cell migration in 3D. These scaffolds allow movement of cells in all directions. The fabrication, imaging, and quantitative analysis method developed here can be used to do systematic cell studies in 3D.

original image
Get access to the full text of this article