Advanced Materials

Exploring Optical Properties of Liquid Crystals for Developing Label-Free and High-Throughput Microfluidic Immunoassays

Authors

  • Chang-Ying Xue,

    1. Department of Chemical and Biomolecular Engineering National University of Singapore 4 Engineering Drive 4, Singapore 117576 (Singapore)
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  • Saif A. Khan,

    1. Department of Chemical and Biomolecular Engineering National University of Singapore 4 Engineering Drive 4, Singapore 117576 (Singapore)
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  • Kun-Lin Yang

    Corresponding author
    1. Department of Chemical and Biomolecular Engineering National University of Singapore 4 Engineering Drive 4, Singapore 117576 (Singapore)
    • Department of Chemical and Biomolecular Engineering National University of Singapore 4 Engineering Drive 4, Singapore 117576 (Singapore).
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  • We would like to thank Suhanya Duraiswamy and Pravien Parthiban for their assistance in master fabrication and initial device-molding experiments.

Abstract

The orientational transition of liquid crystals (LCs) is used as a label-free detection mechanism for immunoassays developed in microfluidic systems. LCs only show bright optical textures (visible to the naked eye) in the line-line intersections in which label-free antibodies bind to their surface-immobilized antigens, suggesting the feasibility of using LCs to detect specific antigen-antibody binding events in a high-throughput and multiplexed manner.

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