Enzyme Directed Templating of Artificial Bone Mineral

Authors

  • Erik D. Spoerke,

    1. Department of Materials Science and Engineering Northwestern University, 2220 N. Campus Dr. Evanston, IL 60208 (USA)
    2. Current address: Electronic and Nanostructured Materials, Sandia National Laboratories, 1515 Eubank SE, Albuquerque, NM 87123 (USA)
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  • Shawn G. Anthony,

    1. Department of Materials Science and Engineering Northwestern University, 2220 N. Campus Dr. Evanston, IL 60208 (USA)
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  • Samuel I. Stupp

    Corresponding author
    1. Department of Materials Science and Engineering Northwestern University, 2220 N. Campus Dr. Evanston, IL 60208 (USA)
    2. Institute for BioNanotechnology in Medicine and Department of Chemistry and Department of Medicine Northwestern University 2220 Campus Drive, Evanston, IL 60208 (USA)
    • Department of Materials Science and Engineering Northwestern University, 2220 N. Campus Dr. Evanston, IL 60208 (USA).
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Abstract

original image

An artificial, in vitro biomineralization process that utilizes a nanofiber gel to as a substrate for biomimetic hydroxyapatite mineralization in three dimensions is presented. The system employs the natural enzyme alkaline phosphatase and a phosphorylated, anionic nanofiber gel matrix to template hydroxyapatite nanocrystals with size, shape, and crystallographic orientation resembling natural bone mineral.

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