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A Facile Synthesis Approach to C8-Functionalized Magnetic Carbonaceous Polysaccharide Microspheres for the Highly Efficient and Rapid Enrichment of Peptides and Direct MALDI-TOF-MS Analysis

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Abstract

Biocompatible C8-functionalized magnetic carbonaceous polysaccharide microspheres are synthesized via a facile, low-cost, and large-scale route, and their use for the enrichment of peptides from protein digest mixtures is presented. The process of enrichment is very simple, quick, and efficient. Peptides loaded onto the C8-functionalized magnetic carbonaceous polysaccharide microspheres can be directly analyzed by MALDI-TOF-MS without prior elution from the microspheres.

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