Communication

Simple Fabrication of Antibody Microarrays on Nonfouling Polymer Brushes with Femtomolar Sensitivity for Protein Analytes in Serum and Blood

  1. Angus Hucknall1,2,
  2. Dong-Hwan Kim1,
  3. Srinath Rangarajan2,
  4. Ryan T. Hill2,
  5. William M. Reichert1,
  6. Ashutosh Chilkoti1,2,*

Article first published online: 2 MAR 2009

DOI: 10.1002/adma.200803125

Issue

Advanced Materials

Advanced Materials

Volume 21, Issue 19, pages 1968–1971, May 18, 2009

Additional Information

How to Cite

Hucknall, A., Kim, D.-H., Rangarajan, S., Hill, R. T., Reichert, W. M. and Chilkoti, A. (2009), Simple Fabrication of Antibody Microarrays on Nonfouling Polymer Brushes with Femtomolar Sensitivity for Protein Analytes in Serum and Blood. Advanced Materials, 21: 1968–1971. doi: 10.1002/adma.200803125

Author Information

  1. 1

    Department of Biomedical Engineering Duke University Box 90281, Durham North Carolina 27708-0281 (USA)

  2. 2

    Center for Biologically Inspired Materials and Material Systems Duke University Durham, NC, 27708 (USA)

*Department of Biomedical Engineering Duke University Box 90281, Durham North Carolina 27708-0281 (USA).

Publication History

  1. Issue published online: 12 MAY 2009
  2. Article first published online: 2 MAR 2009
  3. Manuscript Revised: 29 DEC 2008
  4. Manuscript Received: 24 OCT 2008

Funded by

  • Wallace H. Coulter Foundation

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Keywords:

  • Blood;
  • Nonfouling;
  • Polymer brush;
  • Protein microarray;
  • Serum

Graphical Abstract

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A multianalyte antibody array that is spotted on a poly(oligo(ethylene glycol) methacrylate) brush 100 nm thick, grown on glass via surface-initiated atom transfer radical polymerization, has femtomolar limit-of-detection (LOD) of cytokines in serum and whole blood, and a dynamic range of six orders of magnitude for a range of protein analytes.

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