Communication

Synthetic pH-Responsive Polymers for Protein Transduction

  1. William B. Liechty1,
  2. Rongjun Chen1,*,
  3. Farzin Farzaneh2,
  4. Mahvash Tavassoli2,
  5. Nigel K. H. Slater1

Article first published online: 24 JUL 2009

DOI: 10.1002/adma.200901733

Issue

Advanced Materials

Advanced Materials

Volume 21, Issue 38-39, pages 3910–3914, October 19, 2009

Additional Information

How to Cite

Liechty, W. B., Chen, R., Farzaneh, F., Tavassoli, M. and Slater, N. K. H. (2009), Synthetic pH-Responsive Polymers for Protein Transduction. Adv. Mater., 21: 3910–3914. doi: 10.1002/adma.200901733

Author Information

  1. 1

    Department of Chemical Engineering and Biotechnology University of Cambridge, Pembroke Street, Cambridge CB2 3RA (UK)

  2. 2

    Department of Molecular Medicine Guy's, King's and St Thomas's School of Medicine, King's Denmark Hill Campus Rayne Institute, 123 Coldharbour Lane London SE5 9NU (UK)

*Department of Chemical Engineering and Biotechnology University of Cambridge, Pembroke Street, Cambridge CB2 3RA (UK).

Publication History

  1. Issue published online: 16 OCT 2009
  2. Article first published online: 24 JUL 2009
  3. Manuscript Revised: 8 JUN 2009
  4. Manuscript Received: 25 MAY 2009

Funded by

  • Gates Cambridge Trust
  • Universities UK organizations

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Keywords:

  • Apoptin;
  • pH-responsive polymer;
  • Protein delivery;
  • Transduction
Thumbnail image of graphical abstract

A pH-responsive, endosomal membrane disruptive, metabolite-derived polyamide PP-75 is developed to deliver the MBP-Apoptin fusion protein, which induces tumor-specific apoptosis into human osteogenic sarcoma Saos-2 cells. The intracellular distribution and colocalization of MBP-Apoptin-AF647 (MA-AF649) and PP-75-FITC provide strong evidence that PP-75 both enhances uptake and facilitates cytoplasmic release of MBP-Apoptin.

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