Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a Magnetically Recyclable Protein Separation System

Authors

  • Jaeyun Kim,

    1. National Creative Research Initiative Center for Oxide Nanocrystalline Materials School of Chemical and Biological Engineering Seoul National University Seoul 151-744 (Korea)
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  • Yuanzhe Piao,

    1. National Creative Research Initiative Center for Oxide Nanocrystalline Materials School of Chemical and Biological Engineering Seoul National University Seoul 151-744 (Korea)
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  • Nohyun Lee,

    1. National Creative Research Initiative Center for Oxide Nanocrystalline Materials School of Chemical and Biological Engineering Seoul National University Seoul 151-744 (Korea)
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  • Yong Il Park,

    1. National Creative Research Initiative Center for Oxide Nanocrystalline Materials School of Chemical and Biological Engineering Seoul National University Seoul 151-744 (Korea)
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  • In-Hwan Lee,

    1. School of Chemical and Biological Engineering Seoul National University Seoul 151-744 (Korea)
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  • Jung-Ho Lee,

    1. School of Chemical and Biological Engineering Seoul National University Seoul 151-744 (Korea)
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  • Seung R. Paik,

    1. School of Chemical and Biological Engineering Seoul National University Seoul 151-744 (Korea)
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  • Taeghwan Hyeon

    Corresponding author
    1. National Creative Research Initiative Center for Oxide Nanocrystalline Materials School of Chemical and Biological Engineering Seoul National University Seoul 151-744 (Korea)
    • National Creative Research Initiative Center for Oxide Nanocrystalline Materials School of Chemical and Biological Engineering Seoul National University Seoul 151-744 (Korea).
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Abstract

original image

A magnetically recyclable protein separation system has been designed and fabricated by combining ferrimagnetic magnetite cores and NiO nanoparticles decorated onto the mesoporous silica shell with a high surface area (see figure). The exposed NiO nanoparticles provide for the selective adsorption of the His-tagged protein from the mixed-protein solution, as well as cell lysate, and the magnetic core allows the particles to be separated from the solution by applying an external magnetic field.

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