Advanced Materials

Elastic Fully Three-dimensional Microstructure Scaffolds for Cell Force Measurements

Authors

  • Franziska Klein,

    1. Zoologisches Institut, Zell- und Neurobiologie Universität Karlsruhe (TH) Haid-und-Neu-Straße 9, D-76131 Karlsruhe (Germany)
    2. DFG-Center for Functional Nanostructures (CFN) Universität Karlsruhe (TH) Wolfgang-Gaede-Straße 1, D-76131 Karlsruhe (Germany)
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  • Thomas Striebel,

    1. DFG-Center for Functional Nanostructures (CFN) and Institut für Angewandte Physik Universität Karlsruhe (TH) Wolfgang-Gaede-Straße 1, D-76131 Karlsruhe (Germany)
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  • Joachim Fischer,

    1. DFG-Center for Functional Nanostructures (CFN) and Institut für Angewandte Physik Universität Karlsruhe (TH) Wolfgang-Gaede-Straße 1, D-76131 Karlsruhe (Germany)
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  • Zhongxiang Jiang,

    1. Zoologisches Institut, Zell- und Neurobiologie Universität Karlsruhe (TH) Haid-und-Neu-Straße 9, D-76131 Karlsruhe (Germany)
    2. DFG-Center for Functional Nanostructures (CFN) Universität Karlsruhe (TH) Wolfgang-Gaede-Straße 1, D-76131 Karlsruhe (Germany)
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  • Clemens M. Franz,

    1. DFG-Center for Functional Nanostructures (CFN) Universität Karlsruhe (TH) Wolfgang-Gaede-Straße 1, D-76131 Karlsruhe (Germany)
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  • Georg von Freymann,

    1. DFG-Center for Functional Nanostructures (CFN) and Institut für Angewandte Physik Universität Karlsruhe (TH) Wolfgang-Gaede-Straße 1, D-76131 Karlsruhe (Germany)
    2. Institut für Nanotechnologie Forschungszentrum Karlsruhe in der Helmholtz-Gemeinschaft D-76021 Karlsruhe (Germany)
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  • Martin Wegener,

    1. DFG-Center for Functional Nanostructures (CFN) and Institut für Angewandte Physik Universität Karlsruhe (TH) Wolfgang-Gaede-Straße 1, D-76131 Karlsruhe (Germany)
    2. Institut für Nanotechnologie Forschungszentrum Karlsruhe in der Helmholtz-Gemeinschaft D-76021 Karlsruhe (Germany)
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  • Martin Bastmeyer

    Corresponding author
    1. Zoologisches Institut, Zell- und Neurobiologie Universität Karlsruhe (TH) Haid-und-Neu-Straße 9, D-76131 Karlsruhe (Germany)
    • Zoologisches Institut, Zell- und Neurobiologie Universität Karlsruhe (TH) Haid-und-Neu-Straße 9, D-76131 Karlsruhe (Germany).
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Abstract

Elastic 3D scaffolds (see figure) are fabricated into a biocompatible photoresist using direct laser writing. These scaffolds can be rhythmically deformed by single beating cardiomyocytes and calibration with atomic force microscopy indicates that cellular forces down to 10–20 nN are detectable with the setup.

original image

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