Particle/Fluid Interface Replication as a Means of Producing Topographically Patterned Polydimethylsiloxane Surfaces for Deposition of Lipid Bilayers

Authors

  • Anand Bala Subramaniam,

    1. Department of Mechanical and Aerospace Engineering Princeton University Princeton, NJ 08544
    2. School of Engineering and Applied Sciences, Harvard University Cambridge, MA 02138 (USA)
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  • Sigolene Lecuyer,

    1. Department of Mechanical and Aerospace Engineering Princeton University Princeton, NJ 08544
    2. School of Engineering and Applied Sciences, Harvard University Cambridge, MA 02138 (USA)
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  • Kumaran S. Ramamurthi,

    1. Department of Mechanical and Aerospace Engineering Princeton University Princeton, NJ 08544
    2. Laboratory of Molecular Biology, National Cancer Institute Bethesda, MD 20892 (USA)
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  • Richard Losick,

    1. Department of Mechanical and Aerospace Engineering Princeton University Princeton, NJ 08544
    2. Department of Molecular and Cellular Biology, Harvard University Cambridge, MA 02138 (USA)
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  • Howard A. Stone

    Corresponding author
    1. Department of Mechanical and Aerospace Engineering Princeton University Princeton, NJ 08544
    • Department of Mechanical and Aerospace Engineering Princeton University Princeton, NJ 08544.
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Abstract

original image

A simple method to produce topographically patterned substrates from polydimethylsiloxane (PDMS) is presented. Surfaces with positively or negatively curved features, a combination of positively and negatively curved features or geometrically complex biomimetic features are fabricated. High-quality lipid bilayers that conform to the topography of the substrates can be prepared, a characteristic useful for biophysical studies.

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