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Asymmetric DNA Origami for Spatially Addressable and Index-Free Solution-Phase DNA Chips

Authors

  • Zhao Zhang,

    1. Bio-X Center Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education) Shanghai Jiao Tong University Shanghai 200030 (PR China)
    2. Laboratory of Physical Biology, Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 (PR China)
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  • Ying Wang,

    1. Laboratory of Physical Biology, Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 (PR China)
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  • Chunhai Fan,

    Corresponding author
    1. Bio-X Center Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education) Shanghai Jiao Tong University Shanghai 200030 (PR China)
    2. Laboratory of Physical Biology, Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 (PR China)
    • Bio-X Center Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education) Shanghai Jiao Tong University Shanghai 200030 (PR China).
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  • Can Li,

    1. Bio-X Center Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education) Shanghai Jiao Tong University Shanghai 200030 (PR China)
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  • You Li,

    1. Bio-X Center Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education) Shanghai Jiao Tong University Shanghai 200030 (PR China)
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  • Lulu Qian,

    1. Bio-X Center Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education) Shanghai Jiao Tong University Shanghai 200030 (PR China)
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  • Yanming Fu,

    1. Bio-X Center Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education) Shanghai Jiao Tong University Shanghai 200030 (PR China)
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  • Yongyong Shi,

    1. Bio-X Center Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education) Shanghai Jiao Tong University Shanghai 200030 (PR China)
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  • Jun Hu,

    1. Laboratory of Physical Biology, Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 (PR China)
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  • Lin He

    Corresponding author
    1. Bio-X Center Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education) Shanghai Jiao Tong University Shanghai 200030 (PR China)
    • Bio-X Center Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education) Shanghai Jiao Tong University Shanghai 200030 (PR China).
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Abstract

A solution-phase DNA chip is produced using DNA origami-based asymmetric self-assembled tiles incorporating single-stranded DNA linear probes. This DNA origami chip is fully spatially addressable, without having to use position index oligonucleotides. The DNA hybridization is readily detected via atomic force microscopy.

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