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Advanced Materials

Nano-Layered Microneedles for Transcutaneous Delivery of Polymer Nanoparticles and Plasmid DNA

Authors

  • Peter C. DeMuth,

    1. Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA 02139 (USA)
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  • Xingfang Su,

    1. Department of Materials Science and Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA 02139 (USA)
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  • Raymond E. Samuel,

    1. Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA 02139 (USA)
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  • Paula T. Hammond,

    Corresponding author
    1. Department of Chemical Engineering and Institute for Soldier Nanotechnologies, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA 02139 (USA)
    • Department of Chemical Engineering and Institute for Soldier Nanotechnologies, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA 02139 (USA).
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  • Darrell J. Irvine

    Corresponding author
    1. Department of Materials Science and Engineering, Department of Biological Engineering, Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA 02139 (USA), Ragon Institute of MIT, MGH, and Harvard Boston, MA 02139, Howard Hughes Medical Institute, 4000 Jones Bridge Rd., Chevy Chase, MD
    • Department of Materials Science and Engineering, Department of Biological Engineering, Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA 02139 (USA), Ragon Institute of MIT, MGH, and Harvard Boston, MA 02139, Howard Hughes Medical Institute, 4000 Jones Bridge Rd., Chevy Chase, MD
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Abstract

Multilayer-coated microneedles achieve transcutaneous delivery of plasmid DNA to the viable epidermis. Cy3-labeled plasmid DNA encoding luciferase (yellow) is deposited on biodegradable microneedle arrays through multilayer self-assembly and then delivered to the skin by microneedle application to achieve colocalization with Langerhans dendritic cells (MHC II-GFP–green).

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