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Endothelial Cell Guidance in 3D Patterned Scaffolds

Authors

  • Yukie Aizawa,

    1. The Donnelly Centre, University of Toronto, 160 College Street, Toronto, ON M5S 3E1 (Canada)
    2. Department of Chemical Engineering and Applied Chemistry, University of Toronto, 160 College Street, Toronto, ON M5S 3E1 (Canada)
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  • Ryan Wylie,

    1. The Donnelly Centre, University of Toronto, 160 College Street, Toronto, ON M5S 3E1 (Canada)
    2. Department of Chemistry, University of Toronto, 160 College Street, Toronto, ON M5S 3E1 (Canada)
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  • Molly Shoichet

    Corresponding author
    1. The Donnelly Centre, University of Toronto, 160 College Street, Toronto, ON M5S 3E1 (Canada)
    2. Department of Chemical Engineering and Applied Chemistry, University of Toronto, 160 College Street, Toronto, ON M5S 3E1 (Canada)
    3. Department of Chemistry, University of Toronto, 160 College Street, Toronto, ON M5S 3E1 (Canada)
    4. Institute of Biomaterials and Biomedical Engineering, University of Toronto, 160 College Street, Toronto, ON M5S 3E1 (Canada)
    • The Donnelly Centre, University of Toronto, 160 College Street, Toronto, ON M5S 3E1 (Canada).
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Abstract

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Primary endothelial cells are guided in an agarose hydrogel scaffold that is chemically patterned with an immobilized concentration gradient of VEGF165 using multiphoton laser patterning. It is particularly compelling that, in this 3D patterned hydrogel, endothelial cells differentiate to tip and stalk cells, having the morphology that is observed in vivo.

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