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Advanced Materials

Patterned Differentiation of Individual Embryoid Bodies in Spatially Organized 3D Hybrid Microgels

Authors

  • Hao Qi,

    1. Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02115, (USA), Telephone: 1 – 617-768 — 8395, Fax: 1 – 617-768 — 8477
    2. Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, (USA)
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  • Yanan Du,

    1. Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02115, (USA), Telephone: 1 – 617-768 — 8395, Fax: 1 – 617-768 — 8477
    2. Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, (USA)
    3. Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, (China)
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  • Lianyong Wang,

    1. Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02115, (USA), Telephone: 1 – 617-768 — 8395, Fax: 1 – 617-768 — 8477
    2. Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, (USA)
    3. Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin, (China)
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  • Hirokazu Kaji,

    1. Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02115, (USA), Telephone: 1 – 617-768 — 8395, Fax: 1 – 617-768 — 8477
    2. Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, (USA)
    3. Department of Bioengineering and Robotics, Graduate School of Engineering, Tohoku University, Sendai, (Japan)
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  • Hojae Bae,

    1. Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02115, (USA), Telephone: 1 – 617-768 — 8395, Fax: 1 – 617-768 — 8477
    2. Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, (USA)
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  • Ali Khademhosseini

    Corresponding author
    1. Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02115, (USA), Telephone: 1 – 617-768 — 8395, Fax: 1 – 617-768 — 8477
    2. Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, (USA)
    • Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02115, (USA), Telephone: 1 – 617-768 — 8395, Fax: 1 – 617-768 — 8477.
    Search for more papers by this author

Abstract

A microengineered hybrid hydrogel array is developed containing asymmetrical extracellular matrices [polyethylene-glycol (PEG) and gelatin methacrylate (GelMA)] to mimic the anisotropic stem cell niche and induce the patterned differentiation of embryoid bodies (EBs). The asymmetrical matrices around individual aggregate of mouse EBs induce polarity and demonstrate the potential of microscale engineered materials for forming tissues.

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