Cooperation of Biological and Mechanical Signals in Cardiac Progenitor Cell Differentiation

Authors

  • Stefania Pagliari,

    1. Laboratory of Molecular and Cellular Cardiology, Department of Internal Medicine, University of Rome “Tor Vergata” Rome 00133, Italy
    2. Japanese-Italian Tissue Engineering Laboratory (JITEL), Tokyo Women’s Medical University-Waseda, University Joint Institution for Advanced Biomedical Sciences, (TWIns), Tokyo, Japan
    3. Italian Institute for Cardiovascular Research (INRC), 40126 Bologna, Italy
    Current affiliation:
    1. S.P. and A.C.V.S. contributed equally to this work.
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  • Ana Cristina Vilela-Silva,

    1. Instituto de Ciências Biomédicas and Laboratório de Tecido Conjuntivo, Hospital Universitário Clementino Fraga Filho, Rio de Janeiro, Brazil
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  • Giancarlo Forte,

    1. Laboratory of Molecular and Cellular Cardiology, Department of Internal Medicine, University of Rome “Tor Vergata” Rome 00133, Italy
    2. Japanese-Italian Tissue Engineering Laboratory (JITEL), Tokyo Women’s Medical University-Waseda, University Joint Institution for Advanced Biomedical Sciences, (TWIns), Tokyo, Japan
    3. Italian Institute for Cardiovascular Research (INRC), 40126 Bologna, Italy
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  • Francesca Pagliari,

    1. Laboratory of Molecular and Cellular Cardiology, Department of Internal Medicine, University of Rome “Tor Vergata” Rome 00133, Italy
    2. Japanese-Italian Tissue Engineering Laboratory (JITEL), Tokyo Women’s Medical University-Waseda, University Joint Institution for Advanced Biomedical Sciences, (TWIns), Tokyo, Japan
    3. Italian Institute for Cardiovascular Research (INRC), 40126 Bologna, Italy
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  • Corrado Mandoli,

    1. International Research Center for Materials, Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), 1–1 Namiki, Tsukuba, Ibaraki 305–0044, Japan
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  • Giovanni Vozzi,

    1. Interdepartmental Research Center “E. Piaggio”, University of Pisa, 56126 Pisa, Italy
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  • Stefano Pietronave,

    1. Department of Medical Sciences, University “A. Avogadro” of Piemonte Orientale, 28100 Novara, Italy
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  • Maria Prat,

    1. Department of Medical Sciences, University “A. Avogadro” of Piemonte Orientale, 28100 Novara, Italy
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  • Silvia Licoccia,

    1. NAST Centre & Department of Chemical Science and Technology, University of Rome “Tor Vergata” Roma 00133, Italy
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  • Arti Ahluwalia,

    1. Interdepartmental Research Center “E. Piaggio”, University of Pisa, 56126 Pisa, Italy
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  • Enrico Traversa,

    Corresponding author
    1. International Research Center for Materials, Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), 1–1 Namiki, Tsukuba, Ibaraki 305–0044, Japan
    2. NAST Centre & Department of Chemical Science and Technology, University of Rome “Tor Vergata” Roma 00133, Italy
    • International Research Center for Materials, Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), 1–1 Namiki, Tsukuba, Ibaraki 305–0044, Japan.
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  • Marilena Minieri,

    1. Laboratory of Molecular and Cellular Cardiology, Department of Internal Medicine, University of Rome “Tor Vergata” Rome 00133, Italy
    2. Japanese-Italian Tissue Engineering Laboratory (JITEL), Tokyo Women’s Medical University-Waseda, University Joint Institution for Advanced Biomedical Sciences, (TWIns), Tokyo, Japan
    3. Italian Institute for Cardiovascular Research (INRC), 40126 Bologna, Italy
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  • Paolo Di Nardo

    Corresponding author
    1. Laboratory of Molecular and Cellular Cardiology, Department of Internal Medicine, University of Rome “Tor Vergata” Rome 00133, Italy
    2. Japanese-Italian Tissue Engineering Laboratory (JITEL), Tokyo Women’s Medical University-Waseda, University Joint Institution for Advanced Biomedical Sciences, (TWIns), Tokyo, Japan
    3. Italian Institute for Cardiovascular Research (INRC), 40126 Bologna, Italy
    • Laboratory of Molecular and Cellular Cardiology, Department of Internal Medicine, University of Rome “Tor Vergata” Rome 00133, Italy
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Abstract

original image

Three-dimensional cardiac tissue-specific scaffolds made of poly-lactic acid (PLA) have an ordered array of square pores reproducing anisotropic cardiac-like stiffness. The mechano-physical features of the scaffolds favor cardiomyocyte adhesion and survival and combined with biological signals released by neonatal cardiomyocytes can accelerate cardiac stem cell differentiation by emulating in vitro cardiac niche.

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