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Advanced Materials

Protein Tethering into Multiscale Geometries by Covalent Subtractive Printing

Authors

  • Sean R. Coyer,

    1. Woodruff School of Mechanical Engineering, Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332-0363, USA
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  • Emmanuel Delamarche,

    1. IBM Research GmbH, Zurich Research Laboratory, 8803 Rüschlikon, Switzerland
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  • Andrés J. García

    Corresponding author
    1. Woodruff School of Mechanical Engineering, Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332-0363, USA
    • Woodruff School of Mechanical Engineering, Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332-0363, USA.
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Abstract

High-resolution patterns of proteins at multiple length scales are printed by a facile and flexible strategy using a flat elastomeric stamp and direct covalent immobilization onto mixed self-assembled monolayers of tri(ethylene glycol)- and carboxylic acid–hexa(ethylene glycol)-terminated alkanethiols on gold-coated substrates. This is a robust method for generating complex protein arrays with precise control over micrometer- and nanometer-scale pattern geometries while maintaining a protein-adsorption-resistant background.

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