Metal-Enhanced Fluorescence to Quantify Bacterial Adhesion

Authors

  • Kangwon Lee,

    1. School of Engineering and Applied Science and Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA 02138, USA
    Search for more papers by this author
  • Lewis D. Hahn,

    1. School of Engineering and Applied Science and Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA 02138, USA
    Search for more papers by this author
  • William W. Yuen,

    1. School of Engineering and Applied Science and Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA 02138, USA
    Search for more papers by this author
  • Hera Vlamakis,

    1. Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    Search for more papers by this author
  • Roberto Kolter,

    1. Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    Search for more papers by this author
  • David J. Mooney

    Corresponding author
    1. School of Engineering and Applied Science and Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA 02138, USA
    • School of Engineering and Applied Science and Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA 02138, USA.
    Search for more papers by this author

Abstract

original image

A metal-enhanced fluorescence assay enables z-tracking of cell adhesion on surfaces. It is based on a significant enhancement in the fluorescence of labeled bacteria upon approaching a surface and can be used to quantitatively define cell adhesion based on the nanoscale distance between the cells and the surfaces.

Ancillary