Get access

Metal-Enhanced Fluorescence to Quantify Bacterial Adhesion

Authors

  • Kangwon Lee,

    1. School of Engineering and Applied Science and Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA 02138, USA
    Search for more papers by this author
  • Lewis D. Hahn,

    1. School of Engineering and Applied Science and Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA 02138, USA
    Search for more papers by this author
  • William W. Yuen,

    1. School of Engineering and Applied Science and Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA 02138, USA
    Search for more papers by this author
  • Hera Vlamakis,

    1. Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    Search for more papers by this author
  • Roberto Kolter,

    1. Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    Search for more papers by this author
  • David J. Mooney

    Corresponding author
    1. School of Engineering and Applied Science and Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA 02138, USA
    • School of Engineering and Applied Science and Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA 02138, USA.
    Search for more papers by this author

Abstract

original image

A metal-enhanced fluorescence assay enables z-tracking of cell adhesion on surfaces. It is based on a significant enhancement in the fluorescence of labeled bacteria upon approaching a surface and can be used to quantitatively define cell adhesion based on the nanoscale distance between the cells and the surfaces.

Get access to the full text of this article

Ancillary