Advanced Materials

Molding Cell Beads for Rapid Construction of Macroscopic 3D Tissue Architecture

Authors

  • Yukiko T. Matsunaga,

    1. Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan
    2. Life BEANS Center, BEANS Project, 4 – 6-1 Komaba, Meguro-ku, Tokyo 153 – 8505, Japan
    Current affiliation:
    1. Y. T. M and Y. M. contributed equally to this work.
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  • Yuya Morimoto,

    1. Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan
    Current affiliation:
    1. Y. T. M and Y. M. contributed equally to this work.
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  • Shoji Takeuchi

    Corresponding author
    1. Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan
    2. Life BEANS Center, BEANS Project, 4 – 6-1 Komaba, Meguro-ku, Tokyo 153 – 8505, Japan
    • Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan.
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Abstract

A microfluidic system was used to prepare a large number of size-controlled collagen gel beads to form microtissue units, “cell beads”, as tissue building blocks. By stacking cell beads into a doll-shaped silicone chamber, millimeter-thick tissue with uniform cell density was formed rapidly. The bead structure allowed the application into the 3D printing, achieving automated geometrical control of the formed tissues for the fabrication of functional complex tissues.

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