Multiplexed Magnetic Labeling Amplification Using Oligonucleotide Hybridization

Authors

  • Monty Liong,

    1. Center for Systems Biology, Massachusetts General Hospital/Harvard Medical School, 185 Cambridge St, Boston, MA 02114, USA
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  • Carlos Tassa,

    1. Center for Systems Biology, Massachusetts General Hospital/Harvard Medical School, 185 Cambridge St, Boston, MA 02114, USA
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  • Stanley Y. Shaw,

    1. Center for Systems Biology, Massachusetts General Hospital/Harvard Medical School, 185 Cambridge St, Boston, MA 02114, USA
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  • Hakho Lee,

    Corresponding author
    1. Center for Systems Biology, Massachusetts General Hospital/Harvard Medical School, 185 Cambridge St, Boston, MA 02114, USA
    • Center for Systems Biology, Massachusetts General Hospital/Harvard Medical School, 185 Cambridge St, Boston, MA 02114, USA.
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  • Ralph Weissleder

    Corresponding author
    1. Center for Systems Biology, Massachusetts General Hospital/Harvard Medical School, 185 Cambridge St, Boston, MA 02114, USA
    2. Department of Systems Biology, Harvard Medical School, 200 Longwood Ave., Alpert 536, Boston, MA 02115, USA
    • Center for Systems Biology, Massachusetts General Hospital/Harvard Medical School, 185 Cambridge St, Boston, MA 02114, USA.
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Abstract

original image

Oligonucleotide hybridization was used as a cell-labeling method to significantly amplify the loading of magnetic probes onto target cells. The method utilized short oligonucleotides as the binding agents between antibodies and superparamagnetic iron oxide. This method not only enabled multiplexed analysis, but also allowed detection of multiple markers on a single sample containing only scant cell numbers.

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