Advanced Materials

Rapid Construction of Three-Dimensional Multilayered Tissues with Endothelial Tube Networks by the Cell-Accumulation Technique

Authors

  • Akihiro Nishiguchi,

    1. Department of Applied Chemistry, Graduate School of Engineering, Osaka University 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan
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  • Hiroaki Yoshida,

    1. Department of Applied Chemistry, Graduate School of Engineering, Osaka University 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan
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  • Michiya Matsusaki,

    1. Department of Applied Chemistry, Graduate School of Engineering, Osaka University 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan
    2. PRESTO, Japan Science and Technology Agency (JST), 4-1-8 Honcho Kawaguchi Saitama 332-0012, Japan
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  • Mitsuru Akashi

    Corresponding author
    1. Department of Applied Chemistry, Graduate School of Engineering, Osaka University 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan
    • Department of Applied Chemistry, Graduate School of Engineering, Osaka University 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan.
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Abstract

A novel cell-accumulation technique has been developed using highly biocompatible nanofilms by layer-by-layer assembly for the rapid construction of thick layered tissues with a well-controlled layer number and thickness. Furthermore, 3D tissues with highly developed blood capillary networks (over 1 cm2 of layered tissues) were also constructed by sandwiching endothelial cells between the layered tissues. Such a simple and rapid methodology would be useful for tissue engineering and drug assessment.

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