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Keywords:

  • size separation;
  • nanoparticles;
  • viscosity gradients;
  • polyvinylpyrrolidone;
  • rate zonal centrifugation
Thumbnail image of graphical abstract

A novel viscosity gradient, built by stacking aqueous solutions of polyvinylpyrrolidone (PVP), is introduced to achieve precise size separation of nanoparticles. The viscosity gradient is more powerful than the conventional density gradient in separating larger nanoparticles. Nanoparticles that aggregate in PVP solutions are successfully stabilized and separated by size.