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Microdrop Printing of Hydrogel Bioinks into 3D Tissue-Like Geometries

Authors

  • Kris Pataky,

    1. Institute of Microengineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Switzerland
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  • Thomas Braschler,

    1. Institute of Microengineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Switzerland
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  • Andrea Negro,

    1. Institute of Bioengineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Switzerland
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  • Philippe Renaud,

    1. Institute of Microengineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Switzerland
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  • Matthias P. Lutolf,

    Corresponding author
    1. Institute of Bioengineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Switzerland
    • Matthias P. Lutolf, Institute of Bioengineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Switzerland

      Juergen Brugger, Institute of Microengineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Switzerland.

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  • Juergen Brugger

    Corresponding author
    1. Institute of Microengineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Switzerland
    • Matthias P. Lutolf, Institute of Bioengineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Switzerland

      Juergen Brugger, Institute of Microengineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Switzerland.

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Abstract

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An optimized 3D inkjet printing process is demonstrated for structuring alginate into a tissue-like microvasculature capable of supporting physiological flow rates. Optimizing the reaction at the single-droplet level enables wet hydrogel droplets to be stacked, thus overcoming their natural tendancy to spread and coalesce. Live cells can be patterned using this process and it can be extended to a range of other hydrogels.

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